Mechanism and optimized conditions for PEG mediated DNA transfection into plant protoplasts

Abstract

Experimental conditions influencing DNA uptake efficiency by maize protoplasts in polyethyleneglycol (PEG) mediated transfection experiments have been studied systematically. The data provide evidence that the extracellular DNA is precipitated efficiently by combined action of PEG together with divalent cations and DNA is taken up by the plant protoplasts… (More)
DOI: 10.1007/BF00716828

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