Mechanism and application of genetic recombination in herpesviruses

@article{Umene1999MechanismAA,
  title={Mechanism and application of genetic recombination in herpesviruses},
  author={Kenichi Umene},
  journal={Reviews in Medical Virology},
  year={1999},
  volume={9}
}
  • K. Umene
  • Published 1 July 1999
  • Biology, Engineering
  • Reviews in Medical Virology
Herpes simplex virus type 1 (HSV‐1) is a ubiquitous human pathogen that latently infects sensory ganglia and encodes over 80 genes in a 152 kbp DNA genome. This well characterised virus provides a model for analysing genetic recombination in herpesviruses, a fundamental biological process by which new combinations of genetic materials are generated. The frequency of homologous recombination was estimated to be 0·0048–0·007 (0·48%–0·7%)/kb of the HSV‐1 genome, determined using physical markers… 

Rad51 and Rad52 Are Involved in Homologous Recombination of Replicating Herpes Simplex Virus DNA

The results indicate a specific role for Rad51 and Rad52 in recombination of replicating herpes simplex virus 1 DNA, and tandem repeats in the US7 gene remained stable upon serial passage, indicating a high fidelity of recombination in infected cells.

The role of DNA repair in herpesvirus pathogenesis.

Phylogenetic Analysis of Clinical Herpes Simplex Virus Type 1 Isolates Identified Three Genetic Groups and Recombinant Viruses

The sequence diversity of the complete genes coding for glycoproteins G (gG), I (gI), and E (gE), comprising 2.3% of the HSV-1 genome and located within the unique short (US) region, is determined for 28 clinical HSVs isolates inducing oral lesions, genital lesions, or encephalitis.

Endonuclease G, a Candidate Human Enzyme for the Initiation of Genomic Inversion in Herpes Simplex Type 1 Virus*

It is proposed that endonuclease G initiates thea sequence-mediated inversion of the L and S components during HSV-1 DNA replication.

G-quadruplexes may determine the landscape of recombination in HSV-1

A correlation between the HSV-1 recombination landscape and the distribution of G 4-motifs and G4-clusters is suggested, with possible implications for the evolution of DNA viruses.

Diversity of the a sequence of herpes simplex virus type 1 developed during evolution.

The nucleotide sequences of the a sequences of 26 HSV-1 isolates were determined, and the DR4 stretches were classified into three groups, suggesting a close association of theDR4 stretch with HSv-1 diversification.

DNA genome of spontaneously occurring deletion mutants of herpes simplex virus type 1 lacking one copy of the inverted repeat sequences of the L component

Three non-engineered, spontaneously occurring herpes simplex virus type 1 (HSV-1) mutants that have a deletion of approximately 10 kbp were isolated and generated through recombination involving regions around the authentic cleavage site in the a sequence, suggesting an important role of the a sequences in the diversification of herpesviruses.

Divergence and Recombination of Clinical Herpes Simplex Virus Type 2 Isolates

It is suggested as a novel finding that homologous recombination is, as reported earlier for HSV-1 and varicella-zoster virus, a prominent feature in the evolution ofHSV-2.

Recombination in alphaherpesviruses

The analysis of concatemers from cells coinfected by two distinguishable alphaherpesviruses provides an efficient tool to study recombination without the bias introduced by invisible or non‐viable recombinant, and by dominance of a virus over recombinants.
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The finding of recombinants in an arrangement that minimized the number of crossover events suggested the participation of both of two arrangements of the L component of parental DNA (P or IS, and IL or ISL) in the generation of the recombinant.

Herpes simplex virus type 1 DNA replication is specifically required for high-frequency homologous recombination between repeated sequences

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