Mechanically unfolding proteins: the effect of unfolding history and the supramolecular scaffold.

  title={Mechanically unfolding proteins: the effect of unfolding history and the supramolecular scaffold.},
  author={Rebecca C Zinober and David J Brockwell and Godfrey S. Beddard and Anthony Blake and Peter D Olmsted and Sheena E Radford and D Alastair M Smith},
  journal={Protein science : a publication of the Protein Society},
  volume={11 12},
The mechanical resistance of a folded domain in a polyprotein of five mutant I27 domains (C47S, C63S I27)(5)is shown to depend on the unfolding history of the protein. This observation can be understood on the basis of competition between two effects, that of the changing number of domains attempting to unfold, and the progressive increase in the compliance of the polyprotein as domains unfold. We present Monte Carlo simulations that show the effect and experimental data that verify these… CONTINUE READING


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