Mechanically induced c-fos expression is mediated by cAMP in MC3T3-E1 osteoblasts.

  title={Mechanically induced c-fos expression is mediated by cAMP in MC3T3-E1 osteoblasts.},
  author={Jamie Fitzgerald and Millie Hughes-Fulford},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  volume={13 3},
In serum-deprived MC3T3-E1 osteoblasts, mechanical stimulation caused by mild (287 x g) centrifugation induced a 10-fold increase in mRNA levels of the proto-oncogene, c-fos. Induction of c-fos was abolished by the cAMP-dependent protein kinase inhibitor H-89, suggesting that the transient c-fos mRNA increase is mediated by cAMP. Down-regulation of protein kinase C (PKC) activity by chronic TPA treatment failed to significantly reduce c-fos induction, suggesting that TPA-sensitive isoforms of… CONTINUE READING


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