Mechanical strain‐induced NO production by bone cells: a possible role in adaptive bone (re)modeling?

@article{Pitsillides1995MechanicalSN,
  title={Mechanical strain‐induced NO production by bone cells: a possible role in adaptive bone (re)modeling?},
  author={Andrew A. Pitsillides and Simon C. F. Rawlinson and Rosemary F. L. Suswillo and Sandrine Bourrin and Gul Zaman and Lance E. Lan Yon},
  journal={The FASEB Journal},
  year={1995},
  volume={9},
  pages={1614 - 1622}
}
The structural competence of the skeleton is maintained by an adaptive mechanism in which resident bone cells respond to load‐induced strains. To investigate the possible role of the messenger molecule nitric oxide (NO) in this response, we studied NO production in well‐characterized organ culture systems, rat long bone‐derived osteoblast‐like (LOBs) cells, and embryonic chick osteocytes (LOCYs) in monolayer culture. In superfused cancellous bone cores, loading (for 15 min) produces increases… 

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The results indicate that COX-2 and constitutive NOS are important signaling molecules in the anabolic responses of neonatal tibial bone to the micromechanical load in vitro.

Mechanical Strain Stimulates Nitric Oxide Production by Rapid Activation of Endothelial Nitric Oxide Synthase in Osteocytes

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It is established that the predominant NOS isoform expressed in rat long bone periosteal osteoblasts and in a distinct population of cortical bone osteocytes is the endothelial form of NOS (eNOS), with little or no expression of the inducible NOS or neuronal N OS isoforms.

Role of Inducible Nitric Oxide Synthase in Skeletal Adaptation to Acute Increases in Mechanical Loading

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Dynamic mechanical compression influences nitric oxide production by articular chondrocytes seeded in agarose.

Inference experiments infer that alterations in cNOS activity primarily determine the response, and NO appears to be a constituent of mechanotransduction pathways which influence proliferation of bovine chondrocytes seeded within agarose constructs.

Bone's Early Responses to Mechanical Loading Differ in Distinct Genetic Strains of Chick: Selection for Enhanced Growth Reduces Skeletal Adaptability

The results indicate that early phases of the adaptive response to loading differ in different genetic strains of embryonic chick; that skeletal abnormalities which develop in genetically selected, high growth rate chicks may reflect a compromised ability to respond to load; and that load‐induced increases in osteoblastic G6PD activity appear to be closely associated with increased rates of NO release.

Expression of Tenascin‐C in Bones Responding to Mechanical Load

Results presented here indicate that tenascin‐C expression by bone cells is enhanced in the early osteogenic response to loading, which may indicate that the protein acts as a mediator of the mechanically adaptive response of bone cells.

Using cell and organ culture models to analyze responses of bone cells to mechanical stimulation.

The text is expanded to include additions and modifications made to the straining apparatus and update the research cited to support this growing role of cell and organ culture models to analyze responses of bone cells to mechanical stimulation.

Calcium‐channel activation and matrix protein upregulation in bone cells in response to mechanical strain

An important role for L‐type calcium channels and a thapsigargin‐sensitive component in early mechanical strain transduction pathways in osteoblasts is suggested.

Tensile Stress Induces Bone Morphogenetic Protein 4 in Preosteoblastic and Fibroblastic Cells, Which Later Differentiate into Osteoblasts Leading to Osteogenesis in the Mouse Calvariae in Organ Culture

  • M. IkegameOsamu Ishibashi H. Kawashima
  • Biology, Medicine
    Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
  • 2001
Results suggest that BMP‐4 may play a pivotal role by acting as an autocrine and a paracrine factor for recruiting osteoblasts in tensile stress‐induced osteogenesis.
...

References

SHOWING 1-10 OF 160 REFERENCES

Ccuaiuisiiuu 3 ’ . 5 - nuosuot ) hosphate in bone : nuss ’ rs , sd ’ o 1 cstvssuuiisamissn he ) amujmuunusssuohsisto - chenuical technititse

  • 1985

Possible iutvolvemuuemtt of NO i uslumugtt - ulsc - tuotd - mutiation Esir

  • 1988

A (1994) humshiecihiie isoloums sd cyt-loxgenase auth uuitsic-texidle synthase mu iuuilansuuiestjuen

  • Proc. Na:! Aem'ud. Sri ISA 91

The role-of bone cells in iuicm'easiuuguuuetaphvseal hard tissue jul m.uI)udly gronistg rats treated with pu'osmaghamtdimt E2

  • Bone
  • 1987

Cloned auuh

  • 1991

Euudothchitsniderived s’elaxssugfattorrehe-ase on actiu’atsseuuof NMDA sc-cc-pIous suggests role as isttercelluIar messemuger

  • 1988

antl Muuuis-atlem, S (1989) Fommatiout of uuitnc oxide fronu l..argsmuima-cmi IhuuC NS: a transthsss-tsoul mu'thcanisni for stimulation sd mItesolusIelt-guanylats

  • antl Muuuis-atlem, S (1989) Fommatiout of uuitnc oxide fronu l..argsmuima-cmi IhuuC NS: a transthsss-tsoul mu'thcanisni for stimulation sd mItesolusIelt-guanylats

ameul VaIlm-. h) (1983) Nucleotithe seqsmenceof theerat cytopheusmeust--at't imigemueNsic-/s'cc-'tess/cRu's 11

    Oxichatiomu of nitric texitle i cmcsisseusus suul

    • 1993

    Iseehatuomiaced jsiiriui ’ clsiesu uif eesteocytes

    • J . Bone Miner . Rca
    ...