Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner.

@article{Hirata2008MechanicalFF,
  title={Mechanical forces facilitate actin polymerization at focal adhesions in a zyxin-dependent manner.},
  author={Hiroaki Hirata and Hitoshi Tatsumi and Masahiro Sokabe},
  journal={Journal of cell science},
  year={2008},
  volume={121 Pt 17},
  pages={2795-804}
}
We examined the effects of mechanical forces on actin polymerization at focal adhesions (FAs). Actin polymerization at FAs was assessed by introducing fluorescence-labeled actin molecules into permeabilized fibroblasts cultured on fibronectin. When cell contractility was inhibited by the myosin-II inhibitor blebbistatin, actin polymerization at FAs was diminished, whereas alpha(5)beta(1) integrin remained accumulated at FAs. This suggests that actin polymerization at FAs depends on mechanical… CONTINUE READING