Mechanical design of proteins studied by single-molecule force spectroscopy and protein engineering.

  title={Mechanical design of proteins studied by single-molecule force spectroscopy and protein engineering.},
  author={Mariano Carri{\'o}n-V{\'a}zquez and Andr{\'e}s F. Oberhauser and Thomas E. Fisher and Piotr E. Marszalek and Hongbin Li and J. M. Fern{\'a}ndez},
  journal={Progress in biophysics and molecular biology},
  volume={74 1-2},
Mechanical unfolding and refolding may regulate the molecular elasticity of modular proteins with mechanical functions. The development of the atomic force microscopy (AFM) has recently enabled the dynamic measurement of these processes at the single-molecule level. Protein engineering techniques allow the construction of homomeric polyproteins for the precise analysis of the mechanical unfolding of single domains. alpha-Helical domains are mechanically compliant, whereas beta-sandwich domains… CONTINUE READING
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