Mechanical coupling between myosin molecules causes differences between ensemble and single-molecule measurements.

@article{Walcott2012MechanicalCB,
  title={Mechanical coupling between myosin molecules causes differences between ensemble and single-molecule measurements.},
  author={Sam Walcott and David M. Warshaw and Edward P. Debold},
  journal={Biophysical journal},
  year={2012},
  volume={103 3},
  pages={501-10}
}
In contracting muscle, individual myosin molecules function as part of a large ensemble, hydrolyzing ATP to power the relative sliding of actin filaments. The technological advances that have enabled direct observation and manipulation of single molecules, including recent experiments that have explored myosin's force-dependent properties, provide detailed insight into the kinetics of myosin's mechanochemical interaction with actin. However, it has been difficult to reconcile these single… CONTINUE READING