Mechanical compression influences intracellular Ca2+ signaling in chondrocytes seeded in agarose constructs.

  title={Mechanical compression influences intracellular Ca2+ signaling in chondrocytes seeded in agarose constructs.},
  author={S. R. Roberts and M. Knight and D. Lee and D. Bader},
  journal={Journal of applied physiology},
  volume={90 4},
  • S. R. Roberts, M. Knight, +1 author D. Bader
  • Published 2001
  • Medicine, Chemistry
  • Journal of applied physiology
  • Ca2+ signaling forms part of a possible mechanotransduction pathway by which chondrocytes may alter their metabolism in response to mechanical loading. In this study, a well-characterized model system utilizing bovine articular chondrocytes embedded in 4% agarose constructs was used to investigate the effect of physiological mechanical compressive strain applied after 1 and 3 days in culture. The intracellular Ca2+ concentration was measured by use of the ratiometric Ca2+ indicator indo 1-AM… CONTINUE READING
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