Mechanical agitation of very dilute antiserum against IgE has no effect on basophil staining properties

@article{Ovelgnne2005MechanicalAO,
  title={Mechanical agitation of very dilute antiserum against IgE has no effect on basophil staining properties},
  author={J. H. Ovelg{\"o}nne and A. W. J. M. Bol and Wim C. J. Hop and Roeland van Wijk},
  journal={Experientia},
  year={2005},
  volume={48},
  pages={504-508}
}
A previously reported2 effect of mechanically agitated dilutions of antiserum raised against IgE was investigated using the loss of metachromatic staining properties of human basophil leukocytes as a model. A series of 24 blind experiments was performed in which we determined the number of toluidine blue-stainable basophils after incubating with vortexed or non-vortexed dilutions of anti-IgE. Tenfold serial dilutions were used, in the range 1021 to 1030 (6.6×10−26 to 6.6×10−35 M anti-IgE). We… Expand
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