Mechanical agitation of very dilute antiserum against IgE has no effect on basophil staining properties

  title={Mechanical agitation of very dilute antiserum against IgE has no effect on basophil staining properties},
  author={J. H. Ovelg{\"o}nne and A. W. J. M. Bol and Wim C. J. Hop and Roeland van Wijk},
A previously reported2 effect of mechanically agitated dilutions of antiserum raised against IgE was investigated using the loss of metachromatic staining properties of human basophil leukocytes as a model. A series of 24 blind experiments was performed in which we determined the number of toluidine blue-stainable basophils after incubating with vortexed or non-vortexed dilutions of anti-IgE. Tenfold serial dilutions were used, in the range 1021 to 1030 (6.6×10−26 to 6.6×10−35 M anti-IgE). We… 

Inhibition of CD203c membrane up-regulation in human basophils by high dilutions of histamine: a controlled replication study

It is shown that low and high dilutions of histamine inhibit CD203c up-regulation in anti-IgE stimulated basophils, using a strictly standardized flow cytometry protocol and a new dilution/succussion procedure.

Original Research Papers Histamine dilutions modulate basophil activation

High dilutions of histamine may indeed exert an effect on basophil activity and this activity observed by staining basophils with alcian blue was confirmed by flow cytometry.

Histamine dilutions modulate basophil activation

In 3 different types of experiment, it has been shown that high dilutions of histamine may indeed exert an effect on basophil activity, with the greatest inhibitory effects seen at lower levels of stimulation.

Sensitive Flow Cytometric Method to Test Basophil Activation Influenced by Homeopathic Histamine Dilutions

A 3-color flow cytometric method with enhanced accuracy was established to measure the effects from homeopathic solutions in an in-vitro cell system and documented an unequivocally inhibited basophil activation by anti-IgE.

Survey of immuno-allergological ultra high dilution research.

  • B. Poitevin
  • Biology, Medicine
    Homeopathy : the journal of the Faculty of Homeopathy
  • 2015

Immunology and Homeopathy. 2. Cells of the Immune System and Inflammation

This review of literature unknown to most people will give an original and useful insight into the ‘state-of-the-art’ of homeopathy, without final conclusions ‘for’ or ‘against’ this modality.

New pharmacological profiles of loratadine: Effects on platelet aggregation and histamine release from repository cells

The present results suggest a broader profile for loratadine beyond its original role as a non-sedative anti-H1-antihistaminic drug.



Human basophil degranulation triggered by very dilute antiserum against IgE

When human polymorphonuclear basophils are exposed to anti-IgE antibodies, they release histamine from their intracellular granules and change their staining properties, and transmission of the biological information could be related to the molecular organization of water.

Bimodal IgG4-mediated human basophil activation. Role of eosinophils.

This study lends support to the role of IgG4 and eosinophils in anaphylactic reactions by proposing that in the latter concentration range, basophil activation is a two-step process: 1) release by anti-IgG4 of eOSinophil cationic proteins that will, in turn, activate human basophils.

[Effect of homeopathic drugs on the phagocytic activity of human granulocytes. In vitro tests in a controlled single-blind study].

Four homeopathic drug preparations with extract dilutions between D1 and D30 (expression for the homeopathic grade of potencies) and some additives (minerals and animal toxins) were investigated,

In vitro stimulation of human granulocytes and lymphocytes by pico- and femtogram quantities of cytostatic agents.

The influence of a broad concentration range of naturally occurring naphthoquinones and other cytotoxic or cytostatic agents on human granulocytes and lymphocytes was investigated and may give an explanation for the antitumour activity of some plant extracts.

Immunomodulatory activity of low doses of interferon alpha,beta in mice.

The study suggests that very low doses of IFN alpha,beta have immunopharmacologic activity, and cytotoxic response of NK cells was stimulated by 5 injections of 2 IU of IFn in both strains of mice.

The Influence of the Homoeopathic Remedy Plumbum Metallicum on the Excretion Kinetics of Lead in Rats

An experiment on the effect of homoeopathically diluted lead on urinary lead excretion in the rat, controlled against distilled water and the chelating agent DMPS found that homoeopathic treatment did not cause a significant change in urinaryLead excretion compared to distilled water, although there were significant differences between different homoeopathy dilutions.

A Study of the Effect of Decimal and Centesimal Dilutions of Arsenic on the Retention and Mobilization of Arsenic in the Rat

A mathematical model confirmed that Hahnemannian dilutions have biological effects which are a direct function of the degree of dilution, which augmented the elimination of arsenic more than the centesimals.

Inquiry into the Limits of Biological Effects of Chemical Compounds in Tissue Culture, I

Three concentration ranges were designed to investigate the low-dose side of the so-called dose-effect relation of mercuric chloride on the proliferation of a murine lymphoblastic cell strain (MB VIa), and differences in cell growth were statistically significant.