Mechanical Fluctuations of the Membrane–Skeleton Are Dependent on F-Actin ATPase in Human Erythrocytes

@article{Tuvia1998MechanicalFO,
  title={Mechanical Fluctuations of the Membrane–Skeleton Are Dependent on F-Actin ATPase in Human Erythrocytes },
  author={Shmuel Tuvia and Shlomo Levin and Arkady Bitler and Rafi Korenstein},
  journal={The Journal of Cell Biology},
  year={1998},
  volume={141},
  pages={1551 - 1561}
}
Cell membrane fluctuations (CMF) of human erythrocytes, measured by point dark field microscopy, were shown to depend, to a large extent, on intracellular MgATP (Levin, S.V., and R. Korenstein. 1991. Biophys. J. 60:733-737). The present study extends that investigation and associates CMF with F-actin's ATPase activity. MgATP was found to reconstitute CMF in red blood cell (RBC) ghosts and RBC skeletons to their levels in intact RBCs, with an apparent Kd of 0.29 mM. However, neither non… CONTINUE READING
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