Mechanical Devices of the Spliceosome: Motors, Clocks, Springs, and Things

@article{Staley1998MechanicalDO,
  title={Mechanical Devices of the Spliceosome: Motors, Clocks, Springs, and Things},
  author={Jonathan P. Staley and Christine Guthrie},
  journal={Cell},
  year={1998},
  volume={92},
  pages={315-326}
}

Figures and Tables from this paper

DEAD-box proteins
The Cell as a Collection Overview of Protein Machines: Preparing the Next Generation of Molecular Biologists
TLDR
It is shown that nearly every major process in a cell is 10 RNA rearrangements as it removes an intron from an carried out by assemblies of 10 or more protein moleRNA transcript, and that protein assemmore elaborate and sophisticated than anything the authors stublies can be enormously complex.
Single-Molecule Pull-Down FRET to Dissect the Mechanisms of Biomolecular Machines.
The spliceosome: caught in a web of shifting interactions.
  • S. Valadkhan
  • Biology
    Current opinion in structural biology
  • 2007
From the Ribosome to the Spliceosome and Back Again
  • C. Guthrie
  • Biology
    The Journal of Biological Chemistry
  • 2009
TLDR
The author chooses to link his earliest beginnings with RNA, as a graduate student studying the ribosome, to his later adventures in what hence became known as “the RNA World,” as a tenured professor studying the spliceosome.
Drosophila MFAP1 Is Required for Pre-mRNA Processing and G2/M Progression*
TLDR
In vivo data show that cells with reduced levels of dMFAP1 or dPrp38 proliferate more slowly than normal cells and undergo apoptosis, suggesting that the observed G2/M arrest might be a general consequence of interfering with spliceosome function.
RNA Editing Accessory Factors — the Example of mHel61p
TLDR
The current structural, genetic and biochemical knowledge on mHel61p, a putative RNA helicase and/or RNPase, is summarized, and an outlook onto dynamic processes of the editing reaction is provided.
Bicaudal D induces selective dynein‐mediated microtubule minus end‐directed transport
TLDR
The N–terminal portion of human Bicaudal D2 (BICD2) is capable of inducing microtubule minus end‐directed movement independently of the molecular context, which offers a new tool to exploit the relocalization of different cellular components by using appropriate targeting motifs.
Flipping the Switch to an Active Spliceosome
Purification of Drosophila snRNPs and characterization of two populations of functional U1 particles.
TLDR
The purification of U1 snRNP particles from Drosophila nuclear extracts are reported and the characterization of their biochemical properties, polypeptide contents, and splicing activities reinforce the view that their activity and composition, with the exception of the atypical bifunctional U1-A/U2-B" SNF protein, are highly conserved in metazoans.
...
...

References

SHOWING 1-10 OF 146 REFERENCES
Protein Translocation: Tunnel Vision
Molecular Movement inside the Translational Engine
G protein mechanisms: insights from structural analysis.
  • S. Sprang
  • Biology, Chemistry
    Annual review of biochemistry
  • 1997
This review is concerned with the structures and mechanisms of a superfamily of regulatory GTP hydrolases (G proteins). G proteins include Ras and its close homologs, translation elongation factors,
Prp43: An RNA helicase-like factor involved in spliceosome disassembly.
  • J. Arenas, J. Abelson
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1997
TLDR
The results presented indicate that JA1 encodes a pre-mRNA processing factor (Prp) involved in disassembly of spliceosomes after the release of mature mRNA, which is renamed this gene PRP43.
PRP28, a 'DEAD-box' protein, is required for the first step of mRNA splicing in vitro.
TLDR
The isolation of PRP28 was reported, a gene in Saccharomyces cerevisiae whose activity is required for the first step of nuclear mRNA splicing in vivo, and it was demonstrated that it is not a stably associated snRNP protein.
Molecular analysis of the SNF2/SWI2 protein family member MOT1, an ATP-driven enzyme that dissociates TATA-binding protein from DNA
TLDR
The results indicate that MOT1 is targeted to TBP both in vitro and in vivo via amino acids in its nonconserved N terminus, and provides a general framework for understanding how members of the SNF2/SWI2 protein family use ATP to modulate protein-DNA interactions to regulate many diverse processes in cells.
The final stages of spliceosome maturation require Spp2p that can interact with the DEAH box protein Prp2p and promote step 1 of splicing.
TLDR
Results suggest that Spp2p interacts with Prp2p in the spliceosome prior to the first cleavage-ligation reaction, which is an essential protein required for the first RNA cleavage reaction in vivo.
Spliceosome activation by PRP2 ATPase prior to the first transesterification reaction of pre-mRNA splicing
  • S. Kim, R. Lin
  • Biology, Chemistry
    Molecular and cellular biology
  • 1996
TLDR
It is hypothesized that PRP2 functions as a molecular motor, similar to some DExH ATPases in transcription, in the activation of the precatalytic spliceosome for the transesterification reaction.
...
...