Measuring very low density lipoprotein-triglyceride kinetics in man in vivo: how different the various methods really are

@article{Magkos2004MeasuringVL,
  title={Measuring very low density lipoprotein-triglyceride kinetics in man in vivo: how different the various methods really are},
  author={Faidon Magkos and Labros S. Sidossis},
  journal={Current Opinion in Clinical Nutrition and Metabolic Care},
  year={2004},
  volume={7},
  pages={547-555}
}
  • F. Magkos, L. Sidossis
  • Published 1 September 2004
  • Biology
  • Current Opinion in Clinical Nutrition and Metabolic Care
Purpose of reviewThe purpose of this article is to briefly outline the methods that are currently available for the determination of very low density lipoprotein-triglyceride (VLDL-TG) kinetics in man in vivo. Recent findingsA number of novel methodologies have been developed over the years for quantifying VLDL-TG production, clearance, and turnover rates. Besides the splanchnic arteriovenous balance technique, tracer methods with radioactive and, more recently, stable isotopes have been widely… 
Measuring VLDL-triglyceride turnover in humans using ex vivo-prepared VLDL tracer Published, JLR Papers in Press, October 18, 2005.
TLDR
A method using unique, ex vivo labeling of the fatty acid moiety of VLDL-TG followed by intravenous bolus infusion in the same person finds that plasma disappearance of ex vivo-labeled VLDl-TG was comparable to that of in vivo- labels and turnover rates can be safely estimated from the log linear decay of V LDL- TG specific activity.
VLDL-TG kinetics: a dual isotope study for quantifying VLDL-TG pool size, production rates, and fractional oxidation in humans.
TLDR
The data demonstrate that VLDL-TG Ra measured by a biexponential fit to a bolus decay curve correlates well with V LDL- TG Ra measure by a primed continuous infusion, and therefore that a "second" peripheral VLDl-TG compartment with rapid exchange of TG exists.
Reproducibility of stable isotope-labeled tracer measures of VLDL-triglyceride and VLDL-apolipoprotein B-100 kinetics Published, JLR Papers in Press, February 26, 2007.
TLDR
There were no systematic differences in plasma FFA, V LDL-TG, and VLDL-apoB-100 concentrations and kinetics between the two studies, and physiologically meaningful differences in mean values can be obtained with a sample size of 6–10 subjects for paired studies and 12–20 subjects per group for cross-sectional studies.
Stable isotope-labeled tracers for the investigation of fatty acid and triglyceride metabolism in humans in vivo
TLDR
This article focuses on the use of stable isotope-labeled tracers for the quantitative evaluation of major pathways of fatty acid and triglyceride metabolism in humans in vivo.
Energy expenditure, insulin, and VLDL-triglyceride production in humans Published, JLR Papers in Press, July 18, 2006.
TLDR
VLDL kinetics are similar in men and women and that REE and plasma insulin are significant independent predictors of VLDL-TG production, and it is suggested that REe plays a greater role in V LDL- TG production than previously anticipated.
Impact of body composition on very-low-density lipoprotein-triglycerides kinetics.
TLDR
It is found that elevated VLDL-TG production without concomitant increased clearance via oxidation and adipose tissue redeposition contributes to hypertriglyceridemia in UBO women.
Determinants of VLDL-triglycerides production
TLDR
Regulation of hepatic VLDL-TG production involves interplay between systemic FFA delivery, hormonal, and nutritional factors that act in concert with hepatic fatty acid handling to regulate short-term and long-term V LDL- TG production.
Basal and Insulin Mediated VLDL-Triglyceride Kinetics in Type 2 Diabetic Men
TLDR
Ex vivo-labeled VLDL-TG tracers, blood and breath samples, fat biopsies, indirect calorimetry, and body composition measures were applied and it was found that greater basal abdominal VLDl-TG storage may help explain the accumulation of upper-body fat in insulin-resistant individuals.
Increased VLDL-Triglyceride Secretion Precedes Impaired Control of Endogenous Glucose Production in Obese, Normoglycemic Men
TLDR
Basal VLDL-TG secretion rates are increased in normoglycemic but insulin-resistant, obese men, resulting in hypertriglyceridemia.
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    Current opinion in clinical nutrition and metabolic care
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