Measuring transcription dynamics in living cells using a photobleaching approach.


The transcriptional kinetics of RNA polymerase II, the enzyme responsible for mRNA transcription in the nucleoplasm, can be modulated by a variety of factors. It is therefore important to establish experimental systems that will enable the readout of transcription kinetics of specific genes as they occur in real time within individual cells. This can be… (More)
DOI: 10.1016/j.ymeth.2017.04.007


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