Measuring the poise of thiol/disulfide couples in vivo.

Abstract

The reduction potentials (E(h)) for the redox couples GSH/GSSG and cysteine/cystine (Cys/CySS) in plasma are useful indicators of systemic oxidative stress and other medically relevant physiological states. This article describes a sensitive method for determining plasma levels of GSH, GSSG, Cys, and CySS used to calculate the in vivo E(h) values. The… (More)
DOI: 10.1016/j.freeradbiomed.2009.08.021

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