Measuring the mass, density, and size of particles and cells using a suspended microchannel resonator

@article{Godin2007MeasuringTM,
  title={Measuring the mass, density, and size of particles and cells using a suspended microchannel resonator},
  author={Michel Godin and Andrea K. Bryan and Thomas P. Burg and K. L. Babcock and Scott R. Manalis},
  journal={Applied Physics Letters},
  year={2007},
  volume={91},
  pages={123121}
}
We demonstrate the measurement of mass, density, and size of cells and nanoparticles using suspended microchannel resonators. The masses of individual particles are quantified as transient frequency shifts, while the particles transit a microfluidic channel embedded in the resonating cantilever. Mass histograms resulting from these data reveal the distribution of a population of heterogeneously sized particles. Particle density is inferred from measurements made in different carrier fluids… 

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References

SHOWING 1-10 OF 23 REFERENCES

Cell spreading on quartz crystal microbalance elicits positive frequency shifts indicative of viscosity changes

It is shown that viscosity variations of cells can affect the resonance frequency of QCM in the absence of apparent cell desorption.

The Quartz Crystal Microbalance as a Continuous Monitoring Tool for the Study of Endothelial Cell Surface Attachment and Growth

The results indicate that the QCM technique can be used for the study of EC attachment and growth and suggest its potential for the real time study of per unit surface area cell mass distribution dynamics and viscoelastic properties and the cells' responses to stresses or perturbations brought about using biologically active molecules.

THE DISTRIBUTION OF BUOYANT DENSITY OF HUMAN ERYTHROCYTES IN BOVINE ALBUMIN SOLUTIONS.

  • R. LeifJ. Vinograd
  • Physics
    Proceedings of the National Academy of Sciences of the United States of America
  • 1964
With general separation procedures, the order of density of certain types of blood cells has been established and it has also been shown that erythrocytes are heterogeneous in density and that young erythropytes are less dense than old.

Determination of the Biomasses of Small Bacteria at Low Concentrations in a Mixture of Species with Forward Light Scatter Measurements by Flow Cytometry

There was agreement between cell mass obtained by flow cytometry for various bacterial species and cell mass computed from Coulter Counter volume and buoyant density, which validated the standard curve and supported the assumption that cells were aligned in the flow stream.

Automated quantitation of cell density distribution and hyperdense cell fraction in RBC disorders.

A recently developed light-scattering method has been used in the present study to quantitate RBC density distribution, which accurately quantitates both the cell density distribution profile and the fraction of dense, dehydrated cells in various RBC disorders.

Growth and buoyant density of Escherichia coli at very low osmolarities

It was determined that both Ca2+ and Mg2+ could stimulate growth below 40 mosM and E. coli might be defined as an euryhalinic and/or euryosmotic bacterium because of its ability to grow in a wide range of salinities and osmolarities.

Blood.

  • P. Sperryn
  • Medicine, Biology
    British journal of sports medicine
  • 1989
The blood's functions include oxygen transport from lungs to tissues in exchange for waste carbon dioxide, transport of hormones, nutrients and waste products between organs, clotting to seal off haemorrhage and the transport of white cells to combat infection.

Particle size analysis: AAPS workshop report, cosponsored by the Food and Drug Administration and the United States Pharmacopeia

The concepts of particle engineering and dosage form design have become dominant themes in pharmaceutical manufacturing and the diversity of particle treatments, methods of particle size analysis, expression and interpretation of data, and process applications results in complicated and sometimes confusing criteria for selection, adoption, or relevance of the available techniques.

Particle Size Characterization

It must be firmly grasped at the beginning of a discussion of techniques for characterizing the size of fineparticles that for all except spherical fineparticles there is no unique size parameter

Concentration of Plasmodium falciparum-infected erythrocytes by density gradient centrifugation in Percoll.

Percoll, a commercially available product marketed by Pharmacia, to be an excellent medium for the isolation of Plasmodium falciparum in vitro, has been found.