Measuring the force of single protein molecule detachment from surfaces with AFM.

@article{Tsapikouni2010MeasuringTF,
  title={Measuring the force of single protein molecule detachment from surfaces with AFM.},
  author={Theodora S Tsapikouni and Yannis F Missirlis},
  journal={Colloids and surfaces. B, Biointerfaces},
  year={2010},
  volume={75 1},
  pages={252-9}
}
Atomic force microscopy (AFM) was used to measure the non-specific detachment force of single fibrinogen molecules from glass surfaces. The identification of single unbinding events was based on the characteristics of the parabolic curves, recorded during the stretching of protein molecules. Fibrinogen molecules were covalently bound to Si(3)N(4) AFM tips, previously modified with 3-aminopropyl-dimethyl-ethoxysilane, through a homobifunctional poly(ethylene glycol) linker bearing two… CONTINUE READING