Measuring single-cell gene expression dynamics in bacteria using fluorescence time-lapse microscopy

@article{Young2011MeasuringSG,
  title={Measuring single-cell gene expression dynamics in bacteria using fluorescence time-lapse microscopy},
  author={Jonathan W. Young and James C W Locke and Alphan Altinok and Nitzan Rosenfeld and Tigran Bacarian and Peter S. Swain and Eric Mjolsness and Michael B. Elowitz},
  journal={Nature Protocols},
  year={2011},
  volume={7},
  pages={80-88}
}
Quantitative single-cell time-lapse microscopy is a powerful method for analyzing gene circuit dynamics and heterogeneous cell behavior. We describe the application of this method to imaging bacteria by using an automated microscopy system. This protocol has been used to analyze sporulation and competence differentiation in Bacillus subtilis, and to quantify gene regulation and its fluctuations in individual Escherichia coli cells. The protocol involves seeding and growing bacteria on small… CONTINUE READING
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