Measuring protein-protein interactions inside living cells using single color fluorescence correlation spectroscopy. Application to human immunodeficiency virus type 1 integrase and LEDGF/p75.

@article{Maertens2005MeasuringPI,
  title={Measuring protein-protein interactions inside living cells using single color fluorescence correlation spectroscopy. Application to human immunodeficiency virus type 1 integrase and LEDGF/p75.},
  author={Goedele Maertens and Jo Vercammen and Zeger Debyser and Yves Engelborghs},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  year={2005},
  volume={19 8},
  pages={1039-41}
}
Recently we described the interaction of human immunodeficiency virus type 1 (HIV-1)1 integrase (IN) with a cellular protein, lens epithelium-derived growth factor/transcription co-activator p75 (LEDGF/p75). We now present the study of the diffusion behavior of the three independent domains of IN and LEDGF/p75 using fluorescence correlation microscopy (FCM). We show that diffusion in the cell of the different enhanced green fluorescent protein (EGFP) fusion proteins is described by two… CONTINUE READING

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