Measuring local gradients of intramitochondrial [Ca(2+)] in cardiac myocytes during sarcoplasmic reticulum Ca(2+) release.

@article{Lu2013MeasuringLG,
  title={Measuring local gradients of intramitochondrial [Ca(2+)] in cardiac myocytes during sarcoplasmic reticulum Ca(2+) release.},
  author={Xiyuan Lu and Kenneth S. Ginsburg and Sarah L Kettlewell and Julie Bossuyt and Godfrey L. Smith and Donald M. Bers},
  journal={Circulation research},
  year={2013},
  volume={112 3},
  pages={424-31}
}
RATIONALE Mitochondrial [Ca(2+)] ([Ca(2+)](mito)) regulates mitochondrial energy production, provides transient Ca(2+) buffering under stress, and can be involved in cell death. Mitochondria are near the sarcoplasmic reticulum (SR) in cardiac myocytes, and evidence for crosstalk exists. However, quantitative measurements of [Ca(2+)](mito) are limited, and spatial [Ca(2+)](mito) gradients have not been directly measured. OBJECTIVE To directly measure local [Ca(2+)](mito) during normal SR Ca… CONTINUE READING