Measuring image resolution in optical nanoscopy

@article{Nieuwenhuizen2013MeasuringIR,
  title={Measuring image resolution in optical nanoscopy},
  author={Robert Nieuwenhuizen and Keith A. Lidke and Mark Bates and Daniela Leyton Puig and David Gr{\"u}nwald and Sjoerd Stallinga and Bernd Rieger},
  journal={Nature Methods},
  year={2013},
  volume={10},
  pages={557-562}
}
Resolution in optical nanoscopy (or super-resolution microscopy) depends on the localization uncertainty and density of single fluorescent labels and on the sample's spatial structure. Currently there is no integral, practical resolution measure that accounts for all factors. We introduce a measure based on Fourier ring correlation (FRC) that can be computed directly from an image. We demonstrate its validity and benefits on two-dimensional (2D) and 3D localization microscopy images of tubulin… CONTINUE READING
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