Measuring circadian oscillations of cytosolic-free calcium in Arabidopsis thaliana.
@article{Hearn2014MeasuringCO,
title={Measuring circadian oscillations of cytosolic-free calcium in Arabidopsis thaliana.},
author={Timothy J Hearn and Alex A R Webb},
journal={Methods in molecular biology},
year={2014},
volume={1158},
pages={
215-26
}
}Circadian oscillations of cytosolic-free calcium can be measured in plants by observing luminescence of the bioluminescent calcium binding protein aequorin. Here we describe the use of intensified photon-counting CCD cameras to measure circadian oscillations in aequorin bioluminescence from Arabidopsis thaliana.