Measuring aptamer equilibria using gradient micro free flow electrophoresis.

Abstract

Gradient micro free flow electrophoresis (muFFE) was used to observe the equilibria of DNA aptamers with their targets (IgE or HIVRT) across a range of ligand concentrations. A continuous stream of aptamer was mixed online with an increasing concentration of target and introduced into the muFFE device, which separated ligand-aptamer complexes from the… (More)
DOI: 10.1021/ac902877v

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