Measuring T cell receptor and T cell gene expression diversity in antigen-responsive human CD4+ T cells.

@article{Eugster2013MeasuringTC,
  title={Measuring T cell receptor and T cell gene expression diversity in antigen-responsive human CD4+ T cells.},
  author={Anne Eugster and Annett Lindner and Anne-Kristin Heninger and Carmen Wilhelm and Sevina Dietz and Mara Catani and Anette-G. Ziegler and Ezio Bonifacio},
  journal={Journal of immunological methods},
  year={2013},
  volume={400-401},
  pages={13-22}
}
T cells have diversity in TCR, epitope recognition, and cytokine production, and can be used for immune monitoring. Furthermore, clonal expansion of TCR families in disease may provide opportunities for TCR-directed therapies. We developed methodology for sequencing expressed genes of TCR alpha and beta chains from single cells and applied this to vaccine (tetanus-toxoid)-responsive CD4(+) T cells. TCR alpha and beta chains were both successfully sequenced in 1309 (43%) of 3038 CD4(+) T cells… CONTINUE READING
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