Measuring Survival of Adherent Cells with the Colony-Forming Assay.

@article{Crowley2016MeasuringSO,
  title={Measuring Survival of Adherent Cells with the Colony-Forming Assay.},
  author={Lisa C. Crowley and Melinda E. Christensen and Nigel J. Waterhouse},
  journal={Cold Spring Harbor protocols},
  year={2016},
  volume={2016 8},
  pages={pdb.prot087171}
}
Measuring cell death with colorimetric or fluorimetric dyes such as trypan blue and propidium iodide (PI) can provide an accurate measure of the number of dead cells in a population at a specific time; however, these assays cannot be used to distinguish cells that are dying or marked for future death. In many cases it is essential to measure the proliferative capacity of treated cells to provide an indirect measurement of cell death. This can be achieved using the colony-forming assay described… 

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References

SHOWING 1-6 OF 6 REFERENCES

Triggering Apoptosis in Hematopoietic Cells with Cytotoxic Drugs.

Treatment of histiocytic lymphoma cell line U937 with actinomycin D provides an ideal model of drug-induced apoptosis that can also be used as a positive control for comparison with other treatments.

Blocking granule-mediated death by primary human NK cells requires both protection of mitochondria and inhibition of caspase activity

It is shown that hGraB triggers caspase activation via mitochondria-dependent and mitochondrial-independent mechanisms that are activated in a hierarchical manner, and that the combined effects of Bcl-2 and direct casp enzyme inhibition can block cell death induced by h GraB and primary hNK cells.

Senescent cells as a source of inflammatory factors for tumor progression

The knowledge of the SASP and the impact it has on tissue microenvironments and ability to stimulate tumor progression is summarized.

The essence of senescence.

The various features of cellular senescence are reviewed and their contribution to tumor suppression is discussed and the power and limitations of the biomarkers currently used to identify senescent cells in vitro and in vivo are highlighted.

Triggering Death of Adherent Cells with Ultraviolet Radiation.

This protocol describes using a Stratalinker UV cross-linker to trigger UV-induced death of HeLa cells.

Measuring Survival of Hematopoietic Cancer Cells with the Colony-Forming Assay in Soft Agar.

This protocol describes the use of soft agar to compare the colony-forming ability of untreated hematopoietic cells to the colonies formed after being treated with a cytotoxic agent.