Measuring Survival of Adherent Cells with the Colony-Forming Assay.

  title={Measuring Survival of Adherent Cells with the Colony-Forming Assay.},
  author={Lisa C. Crowley and Melinda E. Christensen and Nigel J. Waterhouse},
  journal={Cold Spring Harbor protocols},
  volume={2016 8},
Measuring cell death with colorimetric or fluorimetric dyes such as trypan blue and propidium iodide (PI) can provide an accurate measure of the number of dead cells in a population at a specific time; however, these assays cannot be used to distinguish cells that are dying or marked for future death. In many cases it is essential to measure the proliferative capacity of treated cells to provide an indirect measurement of cell death. This can be achieved using the colony-forming assay described… 

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