Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation

@inproceedings{Gabriel2009MeasuringPM,
  title={Measuring Plasma Membrane Protein Endocytic Rates by Reversible Biotinylation},
  author={Luke R. Gabriel and Zachary H Stevens and Haley E Melikian},
  booktitle={Journal of visualized experiments : JoVE},
  year={2009}
}
Plasma membrane proteins are a large, diverse group of proteins comprised of receptors, ion channels, transporters and pumps. Activity of these proteins is responsible for a variety of key cellular events, including nutrient delivery, cellular excitability, and chemical signaling. Many plasma membrane proteins are dynamically regulated by endocytic trafficking, which modulates protein function by altering protein surface expression. The mechanisms that facilitate protein endocytosis are complex… CONTINUE READING

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