Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking.

@article{Khare2016MeasuringNO,
  title={Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking.},
  author={Sonal Khare and Alexander D Radian and Andrea Dorfleutner and Christian Stehlik},
  journal={Methods in molecular biology},
  year={2016},
  volume={1417},
  pages={131-43}
}
Oligomerization of nod-like receptors (NLRs) can be detected by several biochemical techniques dependent on the stringency of protein-protein interactions. Some of these biochemical methods can be combined with functional assays, such as caspase-1 activity assay. Size exclusion chromatography (SEC) allows separation of native protein lysates into different sized complexes by fast protein liquid chromatography (FPLC) for follow-up analysis. Using co-immunoprecipitation (co-IP), combined with SEC… CONTINUE READING