Measuring Mitochondrial Function in Permeabilized Cells Using the Seahorse XF Analyzer or a Clark‐Type Oxygen Electrode

@article{Divakaruni2014MeasuringMF,
  title={Measuring Mitochondrial Function in Permeabilized Cells Using the Seahorse XF Analyzer or a Clark‐Type Oxygen Electrode},
  author={Ajit S Divakaruni and George W. T. Rogers and Anne N Murphy},
  journal={Current Protocols in Toxicology},
  year={2014},
  volume={60}
}
Measurements of mitochondrial respiration in intact cells can help define metabolism and its dysregulation in fields such as cancer, metabolic disease, immunology, and neurodegeneration. Although cells can be offered various substrates in the assay medium, many cell types can oxidize stored pools of energy substrates. A general bioenergetic profile can therefore be obtained using intact cells, but the inability to control substrate provision to the mitochondria can restrict an in‐depth… Expand
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