Measuring Light-dependent Proton Translocation in Isolated Thylakoids

@inproceedings{Dean2014MeasuringLP,
  title={Measuring Light-dependent Proton Translocation in Isolated Thylakoids},
  author={Rob L Dean},
  year={2014}
}
An improved protocol for measuring light-dependent proton translocation across the membrane of isolated thylakoids is described. The method uses a pH electrode attached to data acquisition software to measure the pH increase in the bathing solution as protons are pumped from the solution to the internal compartments of the thylakoid vesicles. Up to the point of light saturation, the magnitude of proton movement depends on irradiance level. Proton translocation is inhibited in a concentration… CONTINUE READING

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