Measurements of unwinding of lac operator by repressor

Abstract

WE report here measurements of the rates of association and dissociation of the lac repressor and covalently closed λplac DNAs of various degrees of superhelicity, from which we deduce that the binding of repressor unwinds the lac operator by 40° to 90°. This small unwinding angle is inconsistent with binding mechanisms involving considerable disruption of… (More)
DOI: 10.1038/251247a0

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