Measurements of threshold of mitochondrial permeability transition pore opening in intact and permeabilized cells by flash photolysis of caged calcium.

@article{Abramov2011MeasurementsOT,
  title={Measurements of threshold of mitochondrial permeability transition pore opening in intact and permeabilized cells by flash photolysis of caged calcium.},
  author={Andrey Y Abramov and Michael R Duchen},
  journal={Methods in molecular biology},
  year={2011},
  volume={793},
  pages={299-309}
}
Changes in intracellular calcium concentration play a major role both in signal transduction and in cell death. In particular, mitochondrial Ca2+ overload is critically important as a determinant of irreversible cell injury. When accumulated above a threshold, matrix Ca2+ triggers opening of the mitochondrial permeability transition pore (mPTP), initiating… CONTINUE READING