Measurements of drug-protein binding by using immobilized human serum albumin liquid chromatography-mass spectrometry.


An HPLC/MS based method was used for fast and convenient determination of drug plasma-protein interactions in early drug discovery screening by employing a human serum albumin affinity column. Results from this methodology were compared with data from ultrafiltration or dialysis methods, and good agreement was observed. A compound not suitable for… (More)