Measurements and modeling of water transport and osmoregulation in a single kidney cell using optical tweezers and videomicroscopy.

@article{Lcio2003MeasurementsAM,
  title={Measurements and modeling of water transport and osmoregulation in a single kidney cell using optical tweezers and videomicroscopy.},
  author={Aline D. L{\'u}cio and Robson Augusto Souza Santos and Oscar Nassif Mesquita},
  journal={Physical review. E, Statistical, nonlinear, and soft matter physics},
  year={2003},
  volume={68 4 Pt 1},
  pages={041906}
}
With an optical tweezer installed in our optical microscope we grab a single Madin Darby Canine kidney cell and keep it suspended in the medium without touching the glass substrate or other cells. Since the optically trapped cell remains with a closely round shape, we can directly measure its volume by using videomicroscopy with digital image analysis. We submit this cell to a hyperosmotic shock (up-shock) and video record the process: the cell initially shrinks due to osmotic efflux of water… CONTINUE READING