Measurements after Electrophoretic Separation

  title={Measurements after Electrophoretic Separation},
  author={Roger Jozef Wieme},
This chapter focuses on measurements after electrophoretic separation. Lactate dehydrogenase (LDH) was the first enzyme for which the existence of isoenzymes was demonstrated with certainty. In virtually all extracts of tissues from higher animals it is possible to separate by electrophoresis 5 fractions with LDH activity. Markert was able to obtain dissociation into subunits by freezing in 1 M NaCl, and after restoration of the original conditions these recombined to the original units. If… CONTINUE READING