Measurement of wheat gluten and barley hordeins in contaminated oats from Europe, the United States and Canada by Sandwich R5 ELISA

@article{Hernando2008MeasurementOW,
  title={Measurement of wheat gluten and barley hordeins in contaminated oats from Europe, the United States and Canada by Sandwich R5 ELISA},
  author={Alberto Hernando and Jorge R. Mujico and Maria C. Mena and Manuel Lombard{\'i}a and Enrique Méndez},
  journal={European Journal of Gastroenterology \& Hepatology},
  year={2008},
  volume={20},
  pages={545-554}
}
Objectives We have investigated the extent of contamination with wheat, barley, rye or a mixture of these cereals in a large number of grains and commercial oats. We have also attempted to identify the type of cereal contaminant. Methods Sandwich R5 ELISA (using either gliadins or hordeins as standards), western blot, matrix-assisted laser desorption/ionization time-of-flight mass spectrometric and quantitative real-time PCR (Q-PCR) techniques have been used to analyze a total of 134 oats… 
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