Measurement of type I interferon production.

@article{Seeds2011MeasurementOT,
  title={Measurement of type I interferon production.},
  author={Rosalind E. Seeds and Joanna Louise Miller},
  journal={Current protocols in immunology},
  year={2011},
  volume={Chapter 14},
  pages={Unit14.21.1-11}
}
The Basic Protocol in this unit describes measurement of murine interferon (IFN)α/β by intracellular staining for these cytokines and detection by flow cytometry. Alternate protocols detail an enzyme-linked immunoabsorbent assay (ELISA) for IFNα and a biological assay to measure IFN. The FACS assay allows measurement of IFNα/β production by defined cell populations, while ELISA measures secreted IFNα. The bioassay measures functional antiviral activity and is the most sensitive of the assays… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…