Measurement of type I interferon production.

  title={Measurement of type I interferon production.},
  author={Rosalind E. Seeds and Joanna Louise Miller},
  journal={Current protocols in immunology},
  volume={Chapter 14},
The Basic Protocol in this unit describes measurement of murine interferon (IFN)α/β by intracellular staining for these cytokines and detection by flow cytometry. Alternate protocols detail an enzyme-linked immunoabsorbent assay (ELISA) for IFNα and a biological assay to measure IFN. The FACS assay allows measurement of IFNα/β production by defined cell populations, while ELISA measures secreted IFNα. The bioassay measures functional antiviral activity and is the most sensitive of the assays… CONTINUE READING

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