Measurement of the pH of frozen buffer solutions by using pH indicators.

@article{Orii1977MeasurementOT,
  title={Measurement of the pH of frozen buffer solutions by using pH indicators.},
  author={Yutaka Orii and Masashi Morita},
  journal={Journal of biochemistry},
  year={1977},
  volume={81 1},
  pages={
          163-8
        }
}
A method was established to estimate the pH change of several buffers solutions on freezing by using a combination of pH indicators. Among more than 30 buffers solutions examined, almost half exhibited a pH change in the temperature range between freezing point and 220 degrees K; the results were tabulated. Glycerol was found to suppress the pH changes because of its "salt buffer" effect. 

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