Measurement of the degree of coupled isotopic enrichment of different positions in an antibiotic peptide by NMR.

  title={Measurement of the degree of coupled isotopic enrichment of different positions in an antibiotic peptide by NMR.},
  author={A. F. Miller and Laura A. Egan and Craig A. Townsend},
  journal={Journal of magnetic resonance},
  volume={125 1},
An experimental strategy for determining the extent to which multiply isotopically labeled fragments are incorporated intact into relatively complicated compounds of interest is presented. The NMR methods employed are based on isotope-filtered one-dimensional spectra and difference HSQC spectra incorporating a spin echo designed to report on the presence of a second NMR active isotope at a coupled site. They supplement existing methods for determining the extent of isotopic incorporation at… 

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  • L. Kay
  • Chemistry, Physics
    Current opinion in structural biology
  • 1995