Measurement of second messengers in signal transduction: cAMP and inositol phosphates.

@article{Gusovsky2001MeasurementOS,
  title={Measurement of second messengers in signal transduction: cAMP and inositol phosphates.},
  author={Fabi{\'a}n Gusovsky},
  journal={Current protocols in neuroscience},
  year={2001},
  volume={Chapter 7},
  pages={
          Unit7.12
        }
}
  • Fabián Gusovsky
  • Published in
    Current protocols in…
    2001
  • Medicine, Biology
  • cAMP acts as an intracellular mediator of hormone action and the importance of accurate quantitative determination of cAMP levels in cells and tissues is widely recognized. The most utilized procedures for the determination of adenylate cyclase activity in membranes are described here for measuring the conversion of [alpha-(32)P]ATP into [(32)P]cAMP after a two-step chromatographic separation. Also critical in signal transduction is phosphoinositide turnover, which is linked to receptor… CONTINUE READING

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    References

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    Inositol phosphates: synthesis and degradation.