Measurement of protein tyrosine phosphatase activity in single cells by capillary electrophoresis.

@article{Phillips2013MeasurementOP,
  title={Measurement of protein tyrosine phosphatase activity in single cells by capillary electrophoresis.},
  author={Ryan M Phillips and Eric Bair and David S Lawrence and Christopher Eldridge Sims and Nancy L Allbritton},
  journal={Analytical chemistry},
  year={2013},
  volume={85 12},
  pages={6136-42}
}
A fluorescent peptide substrate was used to measure dephosphorylation by protein tyrosine phosphatases (PTP) in cell lysates and single cells and to investigate the effect of environmental toxins on PTP activity in these systems. Dephosphorylation of the substrate by PTPN1 and PTPN2 obeyed Michaelis-Menten kinetics, with KM values of 770 ± 250 and 290 ± 54 nM, respectively. Dose-response curves and IC50 values were determined for the inhibition of these two enzymes by the environmental toxins… CONTINUE READING