Measurement of protein-ligand complex formation.

  title={Measurement of protein-ligand complex formation.},
  author={Peter N. Lowe and Cara K. Vaughan and Tina Daviter},
  journal={Methods in molecular biology},
Experimental approaches to detect, measure, and quantify protein-ligand binding, along with their theoretical bases, are described. A range of methods for detection of protein-ligand interactions is summarized. Specific protocols are provided for a nonequilibrium procedure pull-down assay, for an equilibrium direct binding method and its modification into a… CONTINUE READING