Measurement of protein cytokines in tissue extracts by enzyme-linked immunosorbent assays: application to lipopolysaccharide-induced differential milieu of cytokines.

@article{Matalka2005MeasurementOP,
  title={Measurement of protein cytokines in tissue extracts by enzyme-linked immunosorbent assays: application to lipopolysaccharide-induced differential milieu of cytokines.},
  author={Khalid Z. Matalka and Maha F. Tutunji and Mona Abu-Baker and Yousef Abu Baker},
  journal={Neuro endocrinology letters},
  year={2005},
  volume={26 3},
  pages={231-6}
}
OBJECTIVES Determination of protein cytokines in local tissues would help to evaluate their local role in health, sickness behavior and immune-mediated diseases. Therefore, developing a simple quantitative method of protein cytokines in tissues/organs is highly important. METHODS Mouse tissues were collected following intraperitoneal administration of endotoxin-free PBS or lipopolysaccharide. A mild detergent, 0.1% Igepal, was added in a buffer to enhance cytokines extraction. The tissues… CONTINUE READING