Measurement of protein-DNA interactions in vivo by chromatin immunoprecipitation.

  title={Measurement of protein-DNA interactions in vivo by chromatin immunoprecipitation.},
  author={Hogune Im and Jeffrey A. Grass and Kirby D. Johnson and Meghan E. Boyer and Jing Wu and Emery H. Bresnick},
  journal={Methods in molecular biology},
Elucidating mechanisms controlling nuclear processes requires an understanding of the nucleoprotein structure of genes at endogenous chromosomal loci. Traditional approaches to measuring protein-DNA interactions in vitro have often failed to provide insights into physiological mechanisms. Given that most transcription factors interact with simple DNA sequence motifs, which are abundantly distributed throughout a genome, it is essential to pinpoint the small subset of sites bound by factors in… CONTINUE READING