Measurement of pre- and post-synaptic proteins in cerebral cortex: effects of post-mortem delay

@article{Siew2004MeasurementOP,
  title={Measurement of pre- and post-synaptic proteins in cerebral cortex: effects of post-mortem delay},
  author={Lai Khai Siew and Seth Love and David Dawbarn and G. K. Wilcock and Shelley J. Allen},
  journal={Journal of Neuroscience Methods},
  year={2004},
  volume={139},
  pages={153-159}
}
Assessments of synaptic density in human brain are often based on measurements of synaptic proteins. Little information is available on their post-mortem stability. We have investigated this by ELISAs of the pre-synaptic proteins syntaxin and synaptophysin, and the post-synaptic protein PSD-95, in rat and human cortex. The rat brains were cooled in situ from 37 to 20 or 4 degrees C over 3 h, and then kept at 20 or 4 degrees C for a further 24-72 h, to simulate post-mortem storage at room… CONTINUE READING

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