Measurement of plasma unbound unconjugated bilirubin.

  title={Measurement of plasma unbound unconjugated bilirubin.},
  author={Charles E. Ahlfors},
  journal={Analytical biochemistry},
  volume={279 2},
A method is described for measuring the unconjugated fraction of the unbound bilirubin concentration in plasma by combining the peroxidase method for determining unbound bilirubin with a diazo method for measuring conjugated and unconjugated bilirubin. The accuracy of the unbound bilirubin determination is improved by decreasing sample dilution, eliminating interference by conjugated bilirubin, monitoring changes in bilirubin concentration using diazo derivatives, and correcting for rate… CONTINUE READING

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