Measurement of plasma glycerol specific activity by high performance liquid chromatography to determine glycerol flux.

@article{Judd1998MeasurementOP,
  title={Measurement of plasma glycerol specific activity by high performance liquid chromatography to determine glycerol flux.},
  author={Robert L. Judd and Randy Nelson and Samuel Klein and Michael D Jensen and John M. Miles},
  journal={Journal of lipid research},
  year={1998},
  volume={39 5},
  pages={1106-10}
}
Previous methods for measuring plasma glycerol specific activity (SA) are suboptimal, making the determination of glycerol kinetics in vivo with radiotracers difficult. A new high performance liquid chromatography (HPLC) method is described that permits the accurate and specific measurement of glycerol SA. The method involves isolation of glycerol from plasma and the formation of a tribenzoyl derivative. Glycerol rate of appearance was measured in five human volunteers using both [2-3H]glycerol… CONTINUE READING

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