Measurement of nuclear factor-kappa B translocation on lipopolysaccharide-activated human dendritic cells by confocal microscopy and flow cytometry.

@article{Blaecke2002MeasurementON,
  title={Measurement of nuclear factor-kappa B translocation on lipopolysaccharide-activated human dendritic cells by confocal microscopy and flow cytometry.},
  author={Aline Blaecke and Yves Delneste and Nathalie Herbault and Pascale Jeannin and J Bonnefoy and Alain Beck and J C Aubry},
  journal={Cytometry},
  year={2002},
  volume={48 2},
  pages={71-9}
}
BACKGROUND Nuclear factor kappa B (NF-kappaB) is a ubiquitously expressed transcription factor that regulates cytokine and immunoglobulin (Ig) gene expression. In most cell types, the inactive p50/p65 NF-kappaB heterodimer is located in the cytoplasm, complexed to its IkappaB inhibitory unit. Stimulation of cells by various reagents such as bacterial endotoxin or cytokines leads to a dissociation of NF-kappaB from IkappaB and a rapid translocation of free NF-kappaB to the nucleus. The aim of… CONTINUE READING