Measurement of nonsulfated cholecystokinins

  title={Measurement of nonsulfated cholecystokinins},
  author={Mikkel Askj{\ae}r Agersnap and Jens F Rehfeld},
  journal={Scandinavian Journal of Clinical and Laboratory Investigation},
  pages={424 - 431}
  • M. AgersnapJ. Rehfeld
  • Published 1 August 2014
  • Biology
  • Scandinavian Journal of Clinical and Laboratory Investigation
Abstract Most proteins undergo posttranslational modifications that govern the function of the protein. In synchrony, correspondingly unmodified proteins that are functionally silent or act differently may also be synthesized. The gut hormone precursor, procholecystokinin (proCCK) is an example of a protein that is heavily modified. An essential modification is O-sulfation of Y77, which is necessary for the gallbladder emptying effect of CCK peptides via the CCKA-receptor. In order to examine… 

20th International Symposium on Regulatory Peptides (REGPEP2014)

  • Á.
  • Biology
    Journal of Molecular Neuroscience
  • 2014
This study reveals that a substantial part of intestinal CCK is non-sulfated, but only little occurs in the brain, which may be due to a longer lasting posttranslational maturation process in neurons.

Analysis of enteroendocrine cell populations in the human colon

It is concluded that enteroendocrine cells of the human colon, like those of other regions and species, exhibit overlapping patterns of hormone colocalisation and that the hormones and their patterns of expression differ between human and other species.

Distribution and characterisation of CCK containing enteroendocrine cells of the mouse small and large intestine

It is concluded that authentic, biologically active, CCK occurs in EEC of the mouse large intestine.

Cholecystokinin expression in tumors: biogenetic and diagnostic implications.

An overview of the cell-specific biogenesis of CCK peptides, and a description of the CCK expression in tumors and of the cholecystokininoma syndrome are presented.

The uncovering and characterization of a CCKoma syndrome in enteropancreatic neuroendocrine tumor patients

A CCKoma syndrome with severe hypersecretion of CCK exists in man and the duodenal ulcer disease and diarrhea with permanently low gastrin in plasma suggest that CCKomas may mimic gastrinoma-like symptoms, because CCK peptides are full agonists of the gastrin/CCK-B receptor.

The distribution and chemical coding of enteroendocrine cells in Trypanosoma cruzi-infected individuals with chagasic megacolon

The data suggests that the denervation and associated chronic inflammation are accompanied by changes in the number and coding of EEC that could contribute to disorders of motility and defence in the chagasic megacolon.



Unsulfated cholecystokinin: An overlooked hormone?

Isolation and characterization of sulphated and nonsulphated forms of cholecystokinin-58 and their action on gallbladder contraction.

A type of correlation indicates that the N-terminal end of CCK-58 partially compensates for the decrease in activity arising from the lack of sulphated tyrosine, which may have a physiological significance.

Phylogeny of the Cholecystokinin/Gastrin Family

  • A. Johnsen
  • Biology
    Frontiers in Neuroendocrinology
  • 1998
Evidence indicates that the transition from nonmammalian to mammalian gastrin may not be as dramatic as first anticipated, and the CCK/gastrin family appears to be represented in most, if not all, chordates, to which group it may also be limited.

Identification of nonsulfated cholecystokinin-58 in canine intestinal extracts and its biological properties.

The presence of CCK(58)(ns) and its ability to selectively stimulate theCCK(B) receptor without stimulation of the CCk(A) receptor suggest that CCK (58) may have unique physiological properties, especially tissues where the nonsulfated peptide can act as a paracrine or neurocrine agent.

The biology of cholecystokinin and gastrin peptides.

Cholecystokinin (CCK) and gastrin together constitute a family of homologous peptide hormones, which are both physiological ligands for the gastrin/CCK-B receptor, whereas the CCK-A receptor binds

The endoproteolytic maturation of progastrin and procholecystokinin

  • J. Rehfeld
  • Biology
    Journal of Molecular Medicine
  • 2006
It is emphasized that prohormone convertases play a decisive but substrate and cell-specific role in the biosynthetic maturation of gastrin and CCK.

Processing-independent analysis of peptide hormones and prohormones in plasma.

PIA-methods have now been developed for a number of prohormones and proteins, and their diagnostic potential appears promising in diagnosis of cardiovascular disease and in several malignancies.

Endorphins are stored in biologically active and inactive forms: isolation of α-N-acetyl peptides

Experiments reveal that in porcine pituitary the C-and C′-fragments are accompanied by substantial quantities of the corresponding α-N-acetyl peptides, which retain the full immunological potency of the parent peptides.


  • G. Dockray
  • Biology, Medicine
    Current opinion in endocrinology, diabetes, and obesity
  • 2012
Resistance to CCK occurs in obesity and may contribute to altered mechanisms regulating food intake, and CCK may also play a role in adaptive responses in pancreatic islets by maintaining and acting as an incretin in certain circumstances.