Measurement of neuropeptides in crustacean hemolymph via MALDI mass spectrometry.

  title={Measurement of neuropeptides in crustacean hemolymph via MALDI mass spectrometry.},
  author={Ruibing Chen and Mingming Ma and Limei Hui and Jian Zhang and Lingjun Li},
  journal={Journal of the American Society for Mass Spectrometry},
  volume={20 4},
Neuropeptides are often released into circulatory fluid (hemolymph) to act as circulating hormones and regulate many physiological processes. However, the detection of these low-level peptide hormones in circulation is often complicated by high salt interference and rapid degradation of proteins and peptides in crude hemolymph extracts. In this study, we systematically evaluated three different neuropeptide extraction protocols and developed a simple and effective hemolymph preparation method… CONTINUE READING


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