Measurement of myostatin concentrations in human serum: Circulating concentrations in young and older men and effects of testosterone administration.

@article{Lakshman2009MeasurementOM,
  title={Measurement of myostatin concentrations in human serum: Circulating concentrations in young and older men and effects of testosterone administration.},
  author={Kishore M. Lakshman and Shalender Bhasin and Christopher V. Corcoran and Lisa A. Collins-Racie and Lioudmila Tchistiakova and Stephen Bradley Forlow and Katie St Ledger and Michael E. Burczynski and Andrew J. Dorner and Edward R Lavallie},
  journal={Molecular and cellular endocrinology},
  year={2009},
  volume={302 1},
  pages={26-32}
}
UNLABELLED Methodological problems, including binding of myostatin to plasma proteins and cross-reactivity of assay reagents with other proteins, have confounded myostatin measurements. Here we describe development of an accurate assay for measuring myostatin concentrations in humans. Monoclonal antibodies that bind to distinct regions of myostatin served as capture and detector antibodies in a sandwich ELISA that used acid treatment to dissociate myostatin from binding proteins. Serum from… CONTINUE READING

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